1985/01/01 by Karl J. Lackner, S W Law, H. Bryan Brewer · 1 citation
Biochemistry, Genetics and Molecular Biology · #Peroxisome Proliferator-Activated Receptors #RNA and protein synthesis mechanisms #RNA Research and Splicing #Biology #Genetics #Gene #genomic DNA #Molecular biology #Intron #Restriction fragment length polymorphism #Restriction fragment #Nucleic acid #DNA #Restriction map #Nucleic acid sequence #Polymerase chain reaction
paper · pdf · doi:10.1093/nar/13.12.4597
openalex publication_date 1985/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. The complete nucleic acid sequence of the apoA-II gene has been determined, establishing that the apoA-II gene is interrupted by three intervening sequences of 182, 293, and 395 bp. The second intron is of particular interest, because it contains a 33 bp sequence of alternating G and T residues very close to the 3' splice site which has the potential to form a left handed Z-helix structure in vivo. A restriction fragment length polymorphism 3' from the apoA-II gene has been detected which may serve as a marker for the long arm of chromosome 1 in linkage analyses.