2012/07/10 by Takahito Ohshiro, Kazuki Matsubara, Makusu Tsutsui +3 · 2 citations
Engineering · Computer Science · Physics and Astronomy · #Nanopore and Nanochannel Transport Studies #Quantum-Dot Cellular Automata #Surface and Thin Film Phenomena #RNA #DNA #Computational biology #Computer science #Biology #Genetics #Gene
paper · pdf · doi:10.1038/srep00501
openalex publication_date 2012/07/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Two paradigm shifts in DNA sequencing technologies-from bulk to single molecules and from optical to electrical detection-are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore devices have been proposed as third-generation DNA-sequencing devices, a significant milestone in these technologies has been attained by demonstrating a novel technique for resequencing DNA using electrical signals. Here we report single-molecule electrical resequencing of DNA and RNA using a hybrid method of identifying single-base molecules via tunneling currents and random sequencing. Our method reads sequences of nine types of DNA oligomers. The complete sequence of 5'-UGAGGUA-3' from the let-7 microRNA family was also identified by creating a composite of overlapping fragment sequences, which was randomly determined using tunneling current conducted by single-base molecules as they passed between a pair of nanoelectrodes.