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Reconstructions of refractive index tomograms via a discrete algebraic reconstruction technique

2017/07/31 by Moosung Lee, Seungwoo Shin, YongKeun Park +1 · 17 citations
Engineering · Physics and Astronomy · #Algebraic Reconstruction Technique #Aperture (computer memory) #Diffraction #Diffraction tomography #Digital Holography and Microscopy #Electrical and Bioimpedance Tomography #Image quality #Iterative reconstruction #Optical Coherence Tomography Applications #Refractive index #Spatial frequency #Tomography #physics.optics

paper · pdf · open access · doi:10.1364/oe.25.027415

published in Optics Express 25(22), 27415 (Optica Publishing Group)

openalex created_date 2017/07/14 · arxiv created 2017/09/19 · openalex publication_date 2017/10/24 · arxiv updated 2017/11/22 · openalex updated_date 2026/08/05

Abstract

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has a limited access to diffracted light and suffers from poor spatial resolution, particularly along the axial direction. To overcome the limitation of the quality of RI tomography, we present an algorithm that accurately reconstructs RI tomography of a specimen with discrete and uniform RI, using prior information about the RI levels. Through simulations and experiments on various samples, including microspheres, red blood cells, and water droplets, we show that the proposed method can precisely reconstruct RI tomograms of samples which have discrete and homogenous RI distributions in the presence of the missing information and noise.

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