1985/01/01 by Robert D. Burgoyne, Kathyrn-Marie Norman · 1 citation
Agricultural and Biological Sciences · Medicine · #Coccidia and coccidiosis research #Peptidase Inhibition and Analysis #Trypanosoma species research and implications #Chromaffin cell #Tropomyosin #Adrenal medulla #Biology #Immunoperoxidase #Molecular biology #Biochemistry #Membrane #Granule (geology) #Actin #Antibody #Catecholamine #Endocrinology #Monoclonal antibody #Immunology
paper · pdf · doi:10.1016/0014-5793(85)80183-6
openalex publication_date 1985/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/26
The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti-tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of Mr 39 000, 36 500 and 31 000 in 100 000 X g supernatants and heat-stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39-kDa and 31-kDa polypeptides comigrated with polypeptides labelled by anti-tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39-kDa polypeptide that was labelled by anti-tropomyosin.