Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals

2023/12/13 by Priti Mudgil, Mouza Khamis Obaid Al Dhaheri, Maitha Saif Mohammed Alsubousi +7 · 27 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Amylase #Bacteria #Biochemistry #Biology #Bovine milk #Chemistry #Docking (animal) #Enzyme #Fermentation #Fermented milk products #Food science #IC50 #In vitro #Lactic acid #Meat and Animal Product Quality #Medicine #Peptide #Probiotic #Probiotics and Fermented Foods #Protein Hydrolysis and Bioactive Peptides #Veterinary medicine

paper · pdf · doi:10.3168/jds.2023-24118

published in Journal of Dairy Science 107(5), 2633-2652 (Elsevier BV)

openalex publication_date 2023/12/13 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Milk derived peptides have emerged as a popular mean to manage various lifestyle disorders such as diabetes. Fermentation is being explored as one of the faster and efficient way of producing peptides with antidiabetic potential. Therefore, in this study an attempt was made to comparatively investigate the α-amylase (AA) inhibitory properties of peptides derived from milk of different farm animals through probiotic fermentation. Peptide's identification was carried out using LC-MS-QTOF and inhibition mechanism were characterized molecular docking. Results obtained showed an AA-IC 50 value between 2.39 and 36.1 µg protein equivalent for different fermented samples. Overall, Pediococcus pentosaceus MF000957 (PPe) derived fermented milk from all animals indicated higher AA inhibition than other probiotic derived fermented milk (AA-IC 50 values of 6.01, 3.53, 15.6 and 10.8 µg protein equivalent for bovine, camel, goat and sheep fermented milk). Further, molecular docking analysis indicated that camel milk derived peptide IMEQQQTEDEQQDK and goat milk derived peptide DQHQKAMKPWTQPK were the most potent AA inhibitory peptides. Overall, the study concluded that fermentation derived peptides may prove useful in for managing diabetes via inhibition of carbohydrate digesting enzyme AA.

Citations

• Improved Method for Determining Food Protein Degree of Hydrolysis

Cited by

• Functional Peptides from Yak Milk Casein: Biological Activities and Structural Characteristics. [europepmc]
• Molecular Modelling in Bioactive Peptide Discovery and Characterisation. [europepmc]
• Bovine Milk Protein-Derived Preparations and Their Hydrolysates as Sources of ACE-Inhibitory, DPP-IV-Inhibitory, and Antioxidative Peptides Analyzed Using in Silico and in Vitro Protocols. [europepmc]
• Freeze-Dried Probiotic Fermented Camel Milk Enriched with Ajwa Date Pulp: Evaluation of Functional Properties, Probiotic Viability, and In Vitro Antidiabetic and Anticancer Activities. [europepmc]
• Ultrasound-assisted fermentation of buttermilk by Limosilactobacillus fermentum and Lacticaseibacillus rhamnosus : Effects on bacterial proliferation, antioxidant potential, and enzymatic inhibition. [europepmc]

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