2001/01/01 by Unang Supratman, Tomoyuki Fujita, Kohki Akiyama +5 · 1 citation
Biochemistry, Genetics and Molecular Biology · Materials Science · Chemistry · Medicine · #Phytochemical Studies and Bioactivities #Phytochemistry and Bioactive Compounds #Natural product bioactivities and synthesis #Kalanchoe #Crassulaceae #Raji cell #Biology #IC50 #Traditional medicine #Biochemistry #Chemistry #In vitro #Botany #Medicine
paper · pdf · doi:10.1271/bbb.65.947
openalex publication_date 2001/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/16
Five bufadienolides (1-5) isolated from the leaves of Kalanchoe pinnata and K. daigremontiana x tubiflora (Crassulaceae) were examined for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation in Raji cells induced by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate. All bufadienolides showed inhibitory activity, and bryophyllin A (1) exhibited the most marked inhibition (IC50 = 0.4 microM) among the tested compounds. Bryophyllin C (2), a reduction analogue of 1, and bersaldegenin-3-acetate (3) lacking the orthoacetate moiety were less active. These results strongly suggest that bufadienolides are potential cancer chemopreventive agents.