Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes

2011/05/01 by Kei Miyamoto, Vincent Pasque, Jérôme Jullien +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Pluripotent Stem Cells Research #Reproductive Biology and Fertility #Genomics and Chromatin Dynamics #Germinal vesicle #Biology #Cell biology #Reprogramming #Chromatin #Oocyte #Genetics #Gene #Embryo

paper · pdf · doi:10.1101/gad.615211

openalex publication_date 2011/05/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/22

Abstract

Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). We also found that an actin signaling protein, Toca-1, enhances Oct4 reactivation by regulating nuclear actin polymerization. Toca-1 overexpression has an effect on the chromatin state of transplanted nuclei, including the enhanced binding of nuclear actin to gene regulatory regions. This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner.

Citations

• Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II
• Nucleoplasmic β-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations
• A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes
• Rapid and Phosphoinositol-Dependent Binding of the SWI/SNF-like BAF Complex to Chromatin after T Lymphocyte Receptor Signaling
• Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization
• Nuclear Translocation of β-Actin Is Involved in Transcriptional Regulation during Macrophage Differentiation of HL-60 Cells
• The auxiliary replicons of Butyrivibrio proteoclasticus : a thesis presented in fulfilment of the Doctorate of Philosophy degree at Massey University, Palmerston North, New Zealand
• Exportin 6: a novel nuclear export receptor that is specific for profilin·actin complexes

Cited by

• Actin [wikipedia]