1978/10/01 by Kazuko Fujiwara, Mary E. Porter, Thomas D. Pollard · 1 citation
Medicine · Biochemistry, Genetics and Molecular Biology · #Cardiomyopathy and Myosin Studies #Cellular Mechanics and Interactions #Viral Infectious Diseases and Gene Expression in Insects #Cytokinesis #Cleavage furrow #Midbody #Biology #Mitosis #Myosin #Cell biology #Cleavage (geology) #Actinin #Cytoplasm #Actin #Staining #Cytoskeleton #Cell #Cell division #Biochemistry #Genetics
paper · pdf · doi:10.1083/jcb.79.1.268
openalex publication_date 1978/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We used antibodies against alpha-actinin and myosin labeled directly with contrasting fluorochromes to localize these contractile proteins simultaneously in dividing chick embryo cells. During mitosis anti-alpha-actinin stains diffusely the entire cytoplasm including the mitotic spindle, while in the same cells intense antimyosin staining delineates the spindle. During cytokinesis both antibodies stain the cleavage furrow intensely, and until the midbody forms the two staining patterns in the same cell are identical at the resolution of the light microscope. Thereafter the anti-alpha-actinin staining of the furrow remains strong, but the antimyosin staining diminishes. These observations suggest that alpha-actinin participates along with actin and myosin in the membrane movements associated with cytokinesis.