1980/01/01 by R. Braun, U. Greeff, K.J. Netter · 2 citations
Pharmacology, Toxicology and Pharmaceutics · Medicine · Chemistry · #Pharmacogenetics and Drug Metabolism #Silymarin and Mushroom Poisoning #Drug-Induced Hepatotoxicity and Protection #Microsome #Phenobarbital #Cytochrome #Chemistry #Metabolite #Hydroxylamine #Microsoma #Dithionite #Metyrapone #Biochemistry #Pharmacology #Endocrinology #Biology #Enzyme
paper · doi:10.3109/00498258009033790
openalex publication_date 1980/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
1. N-Methyl-N-formylhydrazine, formed by hydrolysis from gyromitrin, the main toxin of the edible mushroom Gyromitra esculenta, lowers the cytochrome P-450 concn. in liver microsomes after its application to rats. 2. This decrease can be intensified by pretreatment of the rats with phenobarbital but not by induction with 3-methylcholanthrene. 3. The effect of methylformylhydrazine can be abolished in relation to inhibitor-treated controls by prior administration of SKF 525-A but not metyrapone. 4. After addition of methylformylhydrazine to liver microsomes of rats pretreated with phenobarbital in the presence of a NADPH-regenerating system and O2 a metabolite was formed with a time dependent difference spectral max. at 425 nm. When subsequently the microsomal mixture was reduced by addition of NADPH or sodium dithionite, a new spectrum was obtained with a max. at 447 nm, which decreased within a few minutes with a slight blue-shift. 5. The cytochrome P-450 mediated oxidation of methylformylhydrazine to a hydroxylamine derivative and further to a nitrosamide, is discussed in relation to its importance for the biological action of the hydrazine. This nitrosamide formation may be the reason for the known hepatocarcinogenicity of methylformylhydrazine.