2005/11/16 by Sascha Martens, Iana Parvanova, Jens Zerrahn +4 · 5 citations
Immunology and Microbiology · Medicine · Chemistry · #Toxoplasma gondii Research Studies #Cytomegalovirus and herpesvirus research #Herpesvirus Infections and Treatments #Toxoplasma gondii #GTPase #Vacuole #Cell biology #Chemistry #Biology #Immunology #Cytoplasm
paper · pdf · doi:10.1371/journal.ppat.0010024
openalex publication_date 2005/11/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
The p47 GTPases are essential for interferon-gamma-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We show that the p47 GTPases, including IIGP1, accumulate at vacuoles containing T. gondii. The accumulation is GTP-dependent and requires live parasites. Vacuolar IIGP1 accumulations undergo a maturation-like process accompanied by vesiculation of the parasitophorous vacuole membrane. This culminates in disruption of the parasitophorous vacuole and finally of the parasite itself. Over-expression of IIGP1 leads to accelerated vacuolar disruption whereas a dominant negative form of IIGP1 interferes with interferon-gamma-mediated killing of intracellular parasites. Targeted deletion of the IIGP1 gene results in partial loss of the IFN-gamma-mediated T. gondii growth restriction in mouse astrocytes.