2014/11/25 by Samantha Spindel, Kim E. Sapsford · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · Medicine · #Advanced Biosensing Techniques and Applications #Advanced biosensing and bioanalysis techniques #Bioinformatics #Biology #Biosensor #Biosensors and Analytical Detection #Computational biology #Computer science #Materials science #Medicine #Multiplex #Multiplexing #Nanotechnology #Pathology #Point of care #Telecommunications
paper · pdf · doi:10.3390/s141222313
openalex publication_date 2014/11/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
This review investigates optical sensor platforms for protein multiplexing, the ability to analyze multiple analytes simultaneously. Multiplexing is becoming increasingly important for clinical needs because disease and therapeutic response often involve the interplay between a variety of complex biological networks encompassing multiple, rather than single, proteins. Multiplexing is generally achieved through one of two routes, either through spatial separation on a surface (different wells or spots) or with the use of unique identifiers/labels (such as spectral separation-different colored dyes, or unique beads-size or color). The strengths and weaknesses of conventional platforms such as immunoassays and new platforms involving protein arrays and lab-on-a-chip technology, including commercially-available devices, are discussed. Three major public health concerns are identified whereby detecting medically-relevant markers using Point-of-Care (POC) multiplex assays could potentially allow for a more efficient diagnosis and treatment of diseases.