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Deletion of the MAD2L1 spindle assembly checkpoint gene is tolerated in mouse models of acute T-cell lymphoma and hepatocellular carcinoma

2017/03/20 by Floris Foijer, Lee A. Albacker, Björn Bakker +12 · 1 citation
Medicine · Biochemistry, Genetics and Molecular Biology · #Cancer-related Molecular Pathways #Genomic variations and chromosomal abnormalities #Cancer Genomics and Diagnostics

paper · doi:10.7554/elife.20873

openalex publication_date 2017/03/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

Chromosome instability (CIN) is deleterious to normal cells because of the burden of aneuploidy. However, most human solid tumors have an abnormal karyotype implying that gain and loss of chromosomes by cancer cells confers a selective advantage. CIN can be induced in the mouse by inactivating the spindle assembly checkpoint. This is lethal in the germline but we show here that adult T cells and hepatocytes can survive conditional inactivation of the Mad2l1 SAC gene and resulting CIN. This causes rapid onset of acute lymphoblastic leukemia (T-ALL) and progressive development of hepatocellular carcinoma (HCC), both lethal diseases. The resulting DNA copy number variation and patterns of chromosome loss and gain are tumor-type specific, suggesting differential selective pressures on the two tumor cell types.

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