2022/08/30 by E. V. Kropocheva, Ekaterina Kropocheva, Lidiya Lisitskaya +8
Biochemistry, Genetics and Molecular Biology · #Advanced biosensing and bioanalysis techniques #Argonaute #Biology #CRISPR and Genetic Engineering #Cleave #Computational biology #DNA #Gene #Genetics #Genome #Genome editing #Nucleic acid #RNA #RNA and protein synthesis mechanisms #RNA interference
paper · pdf · doi:10.1134/s0026893322060103
crossref issued 2022/08/30 · crossref published 2022/08/30 · crossref published-online 2022/08/30 · openalex publication_date 2022/08/30 · crossref created 2022/08/30 · crossref published-print 2022/12/01 · openalex created_date 2025/10/10 · crossref deposited 2026/03/29 · crossref indexed 2026/08/03 · openalex updated_date 2026/08/03
Programmable nucleases are the most important tool for manipulating the genes and genomes of both prokaryotes and eukaryotes. Since the end of the 20th century, many approaches were developed for specific modification of the genome. The review briefly considers the advantages and disadvantages of the main genetic editors known to date. The main attention is paid to programmable nucleases from the family of prokaryotic Argonaute proteins. Argonaute proteins can recognize and cleave DNA sequences using small complementary guide molecules and play an important role in protecting prokaryotic cells from invading DNA. Argonaute proteins have already found applications in biotechnology for targeted cleavage and detection of nucleic acids and can potentially be used for genome editing.