Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1995/10/01 by Willem P.C. Stemmer, Andreas Crameri, Kim D. Ha +2 · 3 citations
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #Biology #CRISPR and Genetic Engineering #DNA #DNA ligase #Gene #Gene expression #Genetics #In vitro recombination #Molecular biology #Molecular cloning #Oligonucleotide #Overlap extension polymerase chain reaction #Plasmid #RNA and protein synthesis mechanisms #Restriction enzyme #Transformation (genetics)

paper · doi:10.1016/0378-1119(95)00511-4

crossref issued 1995/10/01 · crossref published 1995/10/01 · crossref published-print 1995/10/01 · openalex publication_date 1995/10/01 · crossref created 2002/07/25 · crossref deposited 2019/04/22 · openalex created_date 2025/10/10 · crossref indexed 2026/06/17 · openalex updated_date 2026/07/15

Citations

• Expression in Escherichia coli of chemically synthesized genes for human insulin.
• DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

Cited by

• DNA Solution of the Maximal Clique Problem
• Large-scale de novo DNA synthesis: technologies and applications
• Polymerase chain reaction [wikipedia]