1996/03/01 by Joel M. Harp, Ed Uberbacher, Arthur E. Roberson +3 · 3 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Materials Science · #Biochemistry #Biology #Biophysics #Chemistry #Crystallography #DNA #DNA Repair Mechanisms #DNA and Nucleic Acid Chemistry #Diffraction #Enzyme Structure and Function #Histone #Histone octamer #Molecular physics #Nucleosome #Optics #Palindrome #Palindromic sequence #Physics #Superstructure #Thermodynamics
paper · doi:10.1107/s0907444995009139
openalex publication_date 1996/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Nucleosome core particles containing a DNA palindrome and purified chicken erythrocyte histone octamer have been reconstituted and crystallized. The dyad symmetry of the palindrome extends the dyad symmetry of the histone octamer to result in a twofold symmetric particle. This ensures that the structure determined by X-ray diffraction will yield a true representation of the DNA strand rather than the twofold averaged structure which would result from using a non-palindromic DNA sequence. The crystals provide isotropic diffraction to 3.2 A with observed reflections extending to d spacings of about 2.8 A using a rotating-anode Cu Kalpha X-ray source. Although the DNA palindrome is a factor contributing to the quality of the diffraction data, another significant factor is an improved preparative technique which enriches for correctly phased nucleosome core particles.