vix.ing · top · new · best · stats

Extracting Super-resolution Structures inside a Single Molecule or Overlapped Molecules from One Blurred Image

2019/09/19 by Edward Y. Sheffield, Sheffield, Edward Y.
Biochemistry, Genetics and Molecular Biology · Computer Science · Engineering · #Advanced Fluorescence Microscopy Techniques #FOS: Computer and information sciences #FOS: Electrical engineering #Human-Computer Interaction (cs.HC) #Image Processing Techniques and Applications #Image and Video Processing (eess.IV) #Photoacoustic and Ultrasonic Imaging #cs.HC #eess.IV #electronic engineering #information engineering

paper · pdf · doi:10.48550/arxiv.1909.08758

Edward Y. Sheffield is a pen name of Yaohua Xie ([email protected], [email protected], http://orcid.org/0000-0001-6780-3156)

openalex publication_date 2019/09/19 · arxiv created 2019/12/07 · arxiv updated 2019/12/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional theories, the points' inner details cannot be recovered because the images' high frequency components are removed due to the diffraction-limit. But this study finds an exception, and full information can be extracted from a diffraction-blurred image. In such a "resolvable condition", neither profile nor detail information is damaged by diffraction. Thereby, it can be recovered reversibly by solving equation systems in spatial domain or frequency domain. This condition is tightly relevant to the imaging condition of existing super-resolution techniques. Based on the condition, a method is proposed which can achieve unlimited high resolutions in principle, and its effectiveness is demonstrated by both theoretical analysis and simulation experiments. It can also work without any observed image outside the region of interest. Simulation experiments also show its tolerance to certain level of noise.

Citations

Related