2007/11/13 by Reid A. Brennen, Hongfeng Yin, Kevin Killeen · 1 citation
Engineering · Chemistry · #Microfluidic and Capillary Electrophoresis Applications #Analytical Chemistry and Chromatography #Innovative Microfluidic and Catalytic Techniques Innovation
paper · doi:10.1021/ac0712805
openalex publication_date 2007/11/13 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/23
We report a method for forming a nanoflow liquid chromatography (nano-LC) gradient using a single fluid pump at flow rates below 1 muL/min by passively forming a gradient on a microfluidic device. This device works together with an Agilent HPLC-Chip to perform high-throughput nanoflow liquid chromatography/mass spectrometry (nano-LC/MS). The nanoflow gradient delay time is reduced from several minutes for a commercial LC nanoflow pump to only a few seconds with this microfluidic device, thus shortening the total analysis time and increasing the analysis throughput. With this microfluidic device, a nano-LC solvent delivery system can be greatly simplified and have increased robustness, reliability, reduced waste, and ease of use.