2017/08/01 by Cerrone Cabanos, Miao Wang, Xianlin Han +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Lipid Membrane Structure and Behavior #Ion channel regulation and function #Neuroscience and Neuropharmacology Research
paper · pdf · doi:10.1016/j.celrep.2017.07.034
openalex publication_date 2017/08/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Lipid regulation of ion channels by low-abundance signaling lipids phosphatidylinositol 4,5-bisphosphate (PIP 2 ) and phosphatidic acid (PA) has emerged as a central cellular mechanism for controlling ion channels and the excitability of nerves. A lack of robust assays suitable for facile detection of a lipid bound to a channel has hampered the probing of the lipid binding sites and measuring the pharmacology of putative lipid agonists for ion channels. Here, we show a fluorescent PIP 2 competition assay for detergent-purified potassium channels, including TWIK-1-related K + -channel (TREK-1). Anionic lipids PA and phosphatidylglycerol (PG) bind dose dependently (9.1 and 96 μM, respectively) and agonize the channel. Our assay shows PIP 2 binds with high affinity (0.87 μM) but surprisingly can directly antagonize TREK-1 in liposomes. We propose a model for TREK-1 lipid regulation where PIP 2 can compete with PA and PG agonism based on the affinity of the lipid for a site within the channel.