1988/09/01 by W.H. Stimson · 3 citations
Neuroscience · Biochemistry, Genetics and Molecular Biology · Medicine · #Neuropeptides and Animal Physiology #Estrogen and related hormone effects #Asthma and respiratory diseases
paper · doi:10.1111/j.1365-3083.1988.tb01459.x
openalex publication_date 1988/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/05/21
Human spleen cells were investigated for specific oestrogen binding using oestradiol-17 beta. High affinity, low capacity binding was observed (Kd = 2.5 X 10(-10) M) and the characteristics of the oestrogen receptor identified were typical of this type of species. T lymphocyte subpopulations were prepared by negative selection by means of the monoclonal antibodies Leu 2a and 3a. Oestrogen receptors were only found in T cells of the suppressor/cytotoxic (CD8-positive) subset while helper/inducer lymphocytes (CD4-positive) showed no significant steroid binding by cytosol and nuclear assays. These results are supported by data from experiments into the effects of oestradiol-17 beta on immunoglobulin secretion, where it was apparent that only the suppressor/cytotoxic T-cell subpopulation was responsive to the presence of the steroid.