2013/10/10 by N. F. Cheville, Judith Stasko · 1 citation
Biochemistry, Genetics and Molecular Biology · #Molecular Biology Techniques and Applications #Advanced Electron Microscopy Techniques and Applications
paper · doi:10.1177/0300985813505114
openalex publication_date 2013/10/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/23
Technical improvements in electron microscopy, both instrumental and preparative, permit increasingly accurate analyses. Digital images for transmission electron microscopy (TEM) can be processed by software programs that automate tasks and create custom tools that allow for image enhancement for brightness, contrast and coloration; for creation of rectangular, ellipsoidal or irregular area selections; and for measurement of mean area and standard deviation. Sample preparation remains a source of error since organelles and spatial arrangements of macromolecules rapidly change after anoxia. Guidelines for maintaining consistency in preparation, examination and interpretation are presented for different electron microscopy (EM) modalities.