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Comparison of manual and automatic processing of biological samples for electron microscopy

2017/02/08 by Rui Yang, Shuai Wang, Jianli Wang +6

paper · doi:10.1002/jemt.22832

Abstract

Abstract The ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time‐consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post‐staining for electron microscopy. Automated sample processing reduces OsO 4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of their study provide a practical and feasible method for the preparation of biological samples for electron microscopy.

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