2014/11/15 by C. Magnain, Caroline Magnain, A. Castel +15 · 25 citations
Engineering · Medicine · Physics and Astronomy · #Blood flow #Doppler effect #Holography #Laser #Laser Doppler velocimetry #Materials science #Medicine #Narrowband #Optical Imaging and Spectroscopy Techniques #Optics #Photoacoustic and Ultrasonic Imaging #Physics #Thermoregulation and physiological responses #physics.optics
paper · pdf · doi:10.1364/josaa.31.002723
published in Journal of the Optical Society of America A 31(12), 2723 (Optica Publishing Group) · 15 pages
openalex publication_date 2014/11/15 · arxiv created 2014/12/01 · arxiv updated 2014/12/02 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time-averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency-shifted reference beam, permits frequency-selective imaging in the radio frequency range. These Doppler images are acquired with an off-axis Mach-Zehnder interferometer. Microvascular hemodynamic components mapping is performed in the cerebral cortex of the mouse and the eye fundus of the rat with near-infrared laser light without any exogenous marker. These measures are made from a basic inverse-method analysis of local first-order optical fluctuation spectra at low radio frequencies, from 0 Hz to 100 kHz. Local quadratic velocity is derived from Doppler broadenings induced by fluid flows, with elementary diffusing wave spectroscopy formalism in backscattering configuration. We demonstrate quadratic mean velocity assessment in the 0.1-10 mm/s range in vitro and imaging of superficial blood perfusion with a spatial resolution of about 10 micrometers in rodent models of cortical and retinal blood flow.