Effects of pH on human bone marrow stromal cells in vitro: Implications for tissue engineering of bone

2002/02/01 by David H. Kohn, Mojgan Sarmadi, Joseph I. Helman +1 · 26 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · Medicine · #Alkaline phosphatase #Biochemistry #Biology #Biomedical engineering #Biophysics #Bone Metabolism and Diseases #Bone Tissue Engineering Materials #Bone marrow #Cancer research #Cell biology #Cell culture #Chemistry #Endocrinology #Enzyme #Extracellular #Extracellular matrix #Immunology #In vitro #Medicine #Molecular biology #Orthopaedic implants and arthroplasty #Osteoblast #Osteocalcin #Procollagen peptidase #Stromal cell #Tissue engineering #Type I collagen

paper · pdf · doi:10.1002/jbm.10050

openalex publication_date 2002/02/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/04

Abstract

The objective of this study was to address the hypothesis that changes in extracellular pH alter collagen gene expression, collagen synthesis, and alkaline phosphatase activity in bone marrow stromal cells (BMSCs). Potential effects of pH on cell function are of particular importance for tissue engineering because considerable effort is being placed on engineering biodegradable polymers that may generate a local acidic microenvironment on degradation. Human and murine single-cell marrow suspensions were plated at a density of 2 x 10(4) cells/cm(2). After 7 days in culture, the pH of the culture medium was adjusted to one of six ranges: > or = 7.8, 7.5.-7.7, 7.2-7.4, 6.9-7.1, 6.6-6.8, or < or = 6.5. After 48 h of exposure to an altered pH, alkaline phosphatase activity and collagen synthesis decreased significantly with decreasing pH. This decrease was two-to threefold as pH decreased from 7.5 to 6.6. In contrast, alpha1(I) procollagen mRNA levels increased two- to threefold as pH was decreased. The trend in osteocalcin mRNA expression was opposite to that of collagen. Small shifts in extracellular pH led to significant changes in the ability of BMSCs to express markers of the osteoblast phenotype. These pH effects potentially relate to the microenvironment supplied by a tissue-engineering scaffold and suggest that degrading polymer scaffolds may influence the biologic activity of the cells in the immediate environment.

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