2001/12/13 by Ron‐Sascha Spitzer, Carsten Perka, Klaus Lindenhayn +3 · 5 citations
Chemistry · Engineering · Medicine · #Alkaline phosphatase #Anatomy #Biochemistry #Biology #Biomedical engineering #Bone Tissue Engineering Materials #Bone and Dental Protein Studies #Cell biology #Chemistry #Composite material #Enzyme #Extracellular matrix #Fibrin #Immunology #Materials science #Matrix (chemical analysis) #Medicine #Mesenchymal stem cell #Molecular biology #Osteocalcin #Periodontal Regeneration and Treatments #Periosteum #Tissue engineering
paper · doi:10.1002/jbm.1277
openalex publication_date 2001/12/13 · openalex created_date 2016/06/24 · openalex updated_date 2026/05/21
Tissue engineering using periosteal cells is a promising approach for bioactive bone repair. Of central importance in tissue engineering is the cell-matrix interaction. In the present study we tested in vitro the influence of alpha-tricalcium phosphate (alpha-TCP) particles on the expression of osteogenic markers in rabbit periosteal cells embedded in specially manufactured fibrin beads. After cell isolation from tibial periosteum of New Zealand White rabbits, and following monolayer culture, cells were embedded in alginate-fibrin beads containing 7.5% alpha-TCP particles and, as a control group, in beads without particles. The alginate was extracted immediately after polymerization. The beads were cultivated for at least 53 days. The DNA content, alkaline phosphatase activity, and osteocalcin level were determined. In monolayer culture the number of cells increased 6.5-fold. DNA content increased in both three-dimensional culture groups but was significantly higher in the beads containing alpha-TCP. Alkaline phosphatase activity increased in both groups without significant differences. Osteocalcin content was significantly higher in the beads containing alpha-TCP than it was in those without alpha-TCP. These observations indicate that matrix engineering using inorganic particles in fibrin culture can influence the osteogenic differentiation of mesenchymal cells. The three-dimensional culture system presented here facilitates the preparation of grafts for bone reconstruction.