2011/06/27 by Thierry Rabilloud, Cécile Lelong · 250 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · #Advanced Biosensing Techniques and Applications #Advanced Proteomics Techniques and Applications #Electrophoresis #Gel electrophoresis #Interfacing #Microfluidic and Capillary Electrophoresis Applications #Proteomics #Sample preparation #Two-dimensional gel electrophoresis #q-bio.GN
paper · pdf · open access · doi:10.1016/j.jprot.2011.05.040
published in Journal of Proteomics 74(10), 1829-1841 (Elsevier BV)
openalex publication_date 2011/06/27 · arxiv created 2011/09/14 · arxiv updated 2011/09/15 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/05
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the process, from sample preparation to in-gel detection of proteins, commenting the constraints and caveats of the technique. Then the limitations and positive features of two-dimensional electrophoresis are discussed (e.g. its unique ability to separate complete proteins and its easy interfacing with immunoblotting techniques), so that the optimal type of applications of this technique in current and future proteomics can be perceived. This is illustrated by a detailed example taken from the literature and commented in detail. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 2).