2008/06/17 by Changyong Song, Huaidong Jiang, Adrian Mancuso +9 · 1 citation
Biochemistry, Genetics and Molecular Biology · Physics and Astronomy · #Advanced Electron Microscopy Techniques and Applications #Advanced X-ray Imaging Techniques #Biology #Capsid #Cell biology #Coherent diffraction imaging #Diffraction #Materials science #Optics #Organelle #Phase retrieval #Physics #Virology #Virus #X-ray #X-ray Spectroscopy and Fluorescence Analysis #physics.bio-ph #physics.optics
paper · pdf · doi:10.1103/physrevlett.101.158101
16 pages, 5 figures
arxiv created 2008/06/17 · openalex publication_date 2008/10/07 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles. Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes.