2019/04/30 by Wonduck Kim, Sung‐Hyun Yang, Mi-Jeong Park +3 · 1 citation
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Genomics and Phylogenetic Studies #Microbial Community Ecology and Physiology #Methane Hydrates and Related Phenomena
paper · doi:10.1099/ijsem.0.003404
openalex publication_date 2019/04/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
A Gram-stain-negative oval-rod-shaped, spore-forming anaerobic bacterium, designated as strain MCWD5T, was isolated from sediment of a salt pond in the Republic of Korea (35° 7′ 18″ N 126° 19′ 4″ E). The 16S rRNA gene sequence analysis revealed that strain MCWD5T had low similarity values to members in the family Lachnospiraceae , such as Robinsoniella peoriensis PPC31T (94.8 %), Ruminococcus gauvreauii CCRI-16110T (94.2 %) and Lachnotalea glycerini DLD10T (94.0 %), and its phylogenetic position is unstable. The strain could grow at 20–42 °C (optimum, 38–42 °C), pH 5.5–10.0 (pH 7.0) and with 0–6 % (2.0 %) NaCl. Strain MCWD5T could not use nitrate, nitrite, sulfate or sulfite as electron acceptors. The strain could utilize various carbohydrates, such as arabinose, cellobiose, glucose, etc., and polymers such as pectin and starch. The major fatty acids of strain MCWD5T were C14 : 0, C16 : 0, C16 : 1 ω7c, C18 : 1 ω7c DMA and summed feature 8 (C17 : 1 ω8c and/or C17 : 2), which was clearly different from those of related genera. The major polar lipids were diphosphatidyglycerol, phosphatidyglycerol and an unknown phospholipid. Based on the results of phylogenetic, physiologic and chemotaxonomic studies, Anaerosacchariphilus polymeriproducens gen. nov., sp. nov. with the type strain MCWD5T (=KCTC 15595T=DSM 105757T) is proposed in the family Lachnospiraceae .