2009/12/01 by Д. Г. Заварзина, D. G. Zavarzina, T. P. Tourova +5 · 1 citation
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Chromium effects and bioremediation #Genomics and Phylogenetic Studies #Microbial Community Ecology and Physiology
paper · doi:10.1134/s0026261709060095
crossref issued 2009/12/01 · crossref published 2009/12/01 · crossref published-print 2009/12/01 · openalex publication_date 2009/12/01 · crossref created 2009/12/04 · crossref published-online 2009/12/06 · openalex created_date 2025/10/10 · crossref deposited 2026/03/29 · openalex updated_date 2026/07/31 · crossref indexed 2026/07/31
An anaerobic, spore-forming bacterium (strain Z-0521) was isolated from the iron-reducing microbial community enriched from sample of bottom sediments from low-mineralized soda lake Khadyn, Tuva upper Yenisey region (Russia). Cells of strain Z-0521 are motile straight Gram-positive rods, 0.7–1.1 (µm in diameter and 3.0–7.0 µm length. It is a mesophilic halotolerante obligate alkaliphilic bacterium with a pH range for growth 8.5–10.7 (optimum at 9.6–9.7). Utilizes carbohydrates. Peptides, organic acids or alcohols are not utilized. In the presence of mannite strain Z-0521 reduces AQDS, arsenate, selenate and selenite. It is capable of N 2 fixation and has nitrogenase gene nif H. The dominant cellular fatty acids are C 16:0 , C 16:1w7c and C a15 . The G+C content in the DNA is 36.2 mol %. 16S rRNA gene sequencing identified strain Z-0521 as a member of rRNA group 6 of the genus Bacillus . Its closest relatives are B. alkalidiazotrophicus and B. macyae (98.3 and 98.1% sequence similarity). On the basis of physiological properties and genetic analysis, it is proposed that strain Z-0521 T should be assigned to a new species of a new genus, Anaerobacillus alkalilacustre gen. nov., sp. nov. It is also proposed that Bacillus arseniciselenatis, Bacillus macyae and Bacillus alkalidiazotrophicus should be transferred to this new genus, with Anaerobacillus arseniciselenatis (formely Bacillus arseniciselenatis ) as the type species.