Citrate Synthase Gene Comparison, a New Tool for Phylogenetic Analysis, and Its Application for the Rickettsiae
1997/04/01 by Véronique Roux, V. ROUX, Elena Rydkina +5 · 567 citations
Agricultural and Biological Sciences · Immunology and Microbiology · Medicine · #16S ribosomal RNA #Biology #Gene #Genetics #Insect symbiosis and bacterial influences #Microbiology #Phylogenetic tree #Phylogenetics #Rickettsia #Rickettsia conorii #Rickettsia prowazekii #Rickettsia rickettsii #Rickettsia typhi #Rickettsiaceae #Spotted fever #Typhus #Vector-borne infectious diseases #Viral Infections and Vectors #Virology #Virus
paper · doi:10.1099/00207713-47-2-252
published in International Journal of Systematic Bacteriology 47(2), 252-261 (Microbiology Society)
openalex publication_date 1997/04/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Abstract
Using PCR and an automated laser fluorescent DNA sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (gltA) of 28 bacteria belonging to the genus Rickettsia. Comparative sequence analysis showed that most of the spotted fever group (SFG) rickettsiae belonged to one of two subgroups. The first subgroup included Rickettsia massiliae, strain Bar 29, Rickettsia rhipicephali, "Rickettsia aeschlimanni," and Rickettsia montana, which have been isolated only from ticks. The second subgroup was larger and included the majority of the human pathogens and also rickettsiae isolated only from ticks; the members of this subgroup were strain S, Rickettsia africae, "Rickettsia monglotimonae," Rickettsia sibirica, Rickettsia parkeri, Rickettsia conorii, Rickettsia rickettsii, the Thai tick typhus rickettsia, the Israeli tick typhus rickettsia, the Astrakhan fever rickettsia, "Rickettsia slovaca," and Rickettsia japonica. The sequence analysis also showed that the tick-borne organisms Rickettsia helvetica and Rickettsia australis and the mite-borne organism Rickettsia akari were associated with the SFG cluster, that Rickettsia prowazekii and Rickettsia typhi, two representatives of the typhus group, clustered together, and that Rickettsia canada; Rickettsia bellii, and the AB bacterium probably represent three new groups. We compared the phylogenetic trees inferred from citrate synthase gene sequences and from 16S ribosomal DNA (rDNA) sequences. For rickettsial phylogeny, the citrate synthase approach was more suitable, as demonstrated by significant bootstrap values for all of the nodes except those in the larger subgroup defined above. We also compared phylogenetic analysis results obtained in a comparison of the sequences of both genes for all of the representatives of the domain Bacteria for which the gltA sequence was determined. We believe that comparison of gltA sequences could be a complementary approach to 16S rDNA sequencing for inferring bacterial evolution, especially when unstable phylogenetic models are obtained from ribosomal sequences because of high levels of sequence similarity between the bacteria studied.
Citations
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- Acute tick-borne rickettsiosis caused by Rickettsia heilongjiangensis in Russian Far East. [europepmc]
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- Rickettsiaceae and Anaplasmataceae infections in Ixodes ricinus ticks from urban and natural forested areas of Poland. [europepmc]
- Rickettsia buchneri sp. nov., a rickettsial endosymbiont of the blacklegged tick Ixodes scapularis. [europepmc]
- Detection of spotted fever group Rickettsiae in ticks from Zhejiang Province, China. [europepmc]
- Vertical transmission of Bartonella schoenbuchensis in Lipoptena cervi. [europepmc]
- Pathogens vectored by the tick, Dermacentor reticulatus, in endemic regions and zones of expansion in Poland. [europepmc]
- Unique Strain of Rickettsia parkeri Associated with the Hard Tick Dermacentor parumapertus Neumann in the Western United States. [europepmc]
- Detection of bacterial pathogens including potential new species in human head lice from Mali. [europepmc]
- Molecular detection of vector-borne bacteria in bat ticks (Acari: Ixodidae, Argasidae) from eight countries of the Old and New Worlds. [europepmc]
- Detection of pathogens in Dermacentor reticulatus in northwestern Europe: evaluation of a high-throughput array. [europepmc]
- Diagnosis of spotted fever group Rickettsia infections: the Asian perspective. [europepmc]
- Multiple vector-borne pathogens of domestic animals in Egypt. [europepmc]
- Rickettsioses as paradigms of new or emerging infectious diseases. [europepmc]
- Body lice as tools for diagnosis and surveillance of reemerging diseases. [europepmc]
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