Codon influence on protein expression in E. coli correlates with mRNA levels

2016/01/12 by Grégory Boël, Reka R. Letso, Reka Letso +18 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Bacteriophages and microbial interactions #RNA and protein synthesis mechanisms #RNA modifications and cancer

paper · doi:10.1038/nature16509

openalex publication_date 2016/01/12 · crossref created 2016/01/12 · crossref issued 2016/01/13 · crossref published 2016/01/13 · crossref published-online 2016/01/13 · crossref published-print 2016/01/21 · crossref deposited 2022/04/19 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/03 · crossref indexed 2026/08/03

Citations

• Widespread Co-translational RNA Decay Reveals Ribosome Dynamics
• Codon Optimality Is a Major Determinant of mRNA Stability
• Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

Cited by

• OPT: Codon optimize gene sequences for E. coli protein overexpression

Discussions

• The first optimization method (AGhead) focuses solely on the first codons to increase the ratio of adenosine to guanosine. [bsky, 0 points, 0 comments]