2025/01/28 by Daniel Wong, Kam‐Ho Wong, Sunjae Park +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #Genomics and Phylogenetic Studies #RNA and protein synthesis mechanisms
paper · doi:10.1016/j.jmb.2025.168965
openalex publication_date 2025/01/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/03
The ability to overexpress proteins is valuable for biotechnology, but not all sequences are compatible with high yield. We previously analyzed the sequence features and mRNA folding stability of a large data set of 6,384 distinct gene constructs, and developed a model for protein yield. Our OPT.williams.edu server (1) predicts the probability an input sequence will produce protein at a high level when overexpressed in E. coli, and (2) returns optimized synonymous sequences designed to boost protein expression. Here we also present experimental evidence of the high yields of our OPT constructs for eight commercially produced proteins.