2016/03/01 by J. Wilde, Jerzy Wilde, Regina Frączek +5
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Insect and Arachnid Ecology and Behavior #Insect and Pesticide Research #Plant and animal studies
paper · pdf · doi:10.1080/00218839.2016.1211394
crossref issued 2016/03/01 · crossref published 2016/03/01 · crossref published-print 2016/03/01 · openalex publication_date 2016/03/01 · crossref published-online 2016/09/16 · crossref created 2016/09/16 · crossref deposited 2020/08/15 · openalex created_date 2025/10/10 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/31
The effect of sublethal doses of imidacloprid on protein content and activity of proteases on honey bees was analyzed. The study was conducted in three experimental groups: colonies from groups BE-5 and BE-200 were contaminated with 5 and 200 ppb of imidacloprid, respectively, via their food supply (syrup and pollen), while group BE was used as control (untreated). Bee samples were collected 3 and 10 weeks after feeding started. Protein concentration in bee tissue extracts was analyzed with reference: (a) to the dose of imidacloprid; and (b) duration of exposure to the chemical. The average quantity of protein content was significantly higher at the 3-week interval than in the 10-week interval and the bees from control colonies (BE) had significantly higher protein contents than contaminated bees (BE-5 and BE-200), even 3 weeks after feeding with imidacloprid started. Similarly, the activity of proteolytic enzymes (proteases) was found to be dependent on the dose of imidacloprid used, compared to bees from control colonies showing significantly higher activity.