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Development of a hybrid apple population: MAS-based testing for Vf gene detection and preliminary field evaluation of its scab resistance

2026/03/05 by Georgeta Maria Guzu, Ioan Zagrai, Claudiu MOLDOVAN +4 · 1 voice
Agricultural and Biological Sciences · #Fungal Plant Pathogen Control #Plant Physiology and Cultivation Studies #Plant Pathogens and Resistance

paper · doi:10.17221/196/2024-hortsci

openalex publication_date 2026/03/05 · openalex created_date 2026/03/06 · openalex updated_date 2026/06/24

Abstract

The apple scab, caused by<i>Venturia inaequalis</i>, isone ofthe most devastating diseases ofapples because itdrastically affects the aerial parts ofthe tree. Apple breeding programs frequently aim todevelop cultivars exhibiting resistance toscab. Thus, controlled pollinations were performed onapple trees in2021. The experiment was organised into ahybridisation scheme involving six apple cultivars. The scheme consists of: five female genitors (‘Auriu de Bistrița’, ‘Jonathan’, ‘Golden Delicious’, ‘Starkrimson’, and ‘Idared’) and one male genitor (‘Florina’ – donor for the <i>Vf</i>). Toquickly identify the <i>Vf</i> gene inthe early development stage ofprogenies, three pairs ofprimers were used: AM19, AL07, and VFC. The hybrid combinations with the highest percentage ofprogenies inheriting the <i>Vf</i> resistance gene were: ‘Starkrimson’ ‘Florina’ (59.2%), followed by‘Jonathan’ ‘Florina’ (57.1%). The hybrids that inherited the <i>Vf</i> resistance gene have been transferred to the field and are being further examined for their resistance against scab infections in the field, in the proximity of a natural source of the inoculum infection pressure.

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