2022/11/09 by Xiaofei Chen, Xiao-Fei Chen, Xin Zhao +1
Biochemistry, Genetics and Molecular Biology · Engineering · Medicine · #Advanced biosensing and bioanalysis techniques #Biosensors and Analytical Detection #SARS-CoV-2 detection and testing
paper · pdf · doi:10.1007/s00604-022-05533-w
crossref issued 2022/11/09 · crossref published 2022/11/09 · crossref published-online 2022/11/09 · openalex publication_date 2022/11/09 · crossref created 2022/11/09 · crossref published-print 2022/12/01 · crossref deposited 2025/04/09 · openalex created_date 2025/10/10 · crossref indexed 2026/08/01 · openalex updated_date 2026/08/01
The epidemic of infectious diseases caused by contagious pathogens is a life-threatening hazard to the entire human population worldwide. A timely and accurate diagnosis is the critical link in the fight against infectious diseases. Aptamer-based biosensors, the so-called aptasensors, employ nucleic acid aptamers as bio-receptors for the recognition of target pathogens of interest. This review focuses on the design strategies as well as state-of-the-art technologies of aptasensor-based diagnostics for infectious pathogens (mainly bacteria and viruses), covering the utilization of three major signal transducers, the employment of aptamers as recognition moieties, the construction of versatile biosensing platforms (mostly micro and nanomaterial-based), innovated reporting mechanisms, and signal enhancement approaches. Advanced point-of-care testing (POCT) for infectious disease diagnostics are also discussed highlighting some representative ready-to-use devices to address the urgent needs of currently prevalent coronavirus disease 2019 (COVID-19). Pressing issues in aptamer-based technology and some future perspectives of aptasensors are provided for the implementation of aptasensor-based diagnostics into practical application.