A Method for Preparing Oligodeoxynucleotides Containing an Apurinic Site

1990/05/02 by Katrin Groebke, Christian Leumann, Christian J. Leumann · 1 citation
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #Advanced biosensing and bioanalysis techniques #RNA and protein synthesis mechanisms

paper · doi:10.1002/hlca.19900730309

Abstract

Abstract In three steps, 2‐deoxy‐ D ‐ribose has been converted into a phosphoramidite building block bearing a ( t ‐Bu)Me 2 Si protecting group at the OH function of the anomeric centre of the furanose ring. This building block was subsequently incorporated into DNA oligomers of various base sequences using the standard phosphoramidite protocol for automated DNA synthesis. The resulting silyl‐oligomers have been purified by HPLC and selectively desilylated to the corresponding free apurinic DNA sequences. The hexamer d (A‐A‐A‐A‐X‐A) (X representing the apurinic site) which was prepared in this way was characterized by 1 H‐ and 31 P‐NMR spectroscopy. The other sequences as well as their fragments, which formed upon treatment with alkali base, were analyzed by polyacrylamide gel electrophoresis.

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• Detecting the Major Degradation Products of Phosphorothioate Oligonucleotides by Chemical Derivatization and Weak Anion Exchange Chromatography. [europepmc]

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