2024/09/09 by Javier Arcos Hodar, Sascha Jung, Mohamed Soudy +2 · 1 voice
Biochemistry, Genetics and Molecular Biology · #Epigenetics and DNA Methylation #RNA Research and Splicing #Cancer-related gene regulation
paper · doi:10.18632/aging.206105
openalex publication_date 2024/09/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/21
partial reprogramming, only improve a few specific cellular processes. In addition, the molecular mechanisms underlying these approaches are largely unknown, which hinders the design of more holistic cellular rejuvenation strategies. To address this issue, we developed SINGULAR (Single-cell RNA-seq Investigation of Rejuvenation Agents and Longevity), a cell rejuvenation atlas that provides a unified system biology analysis of diverse rejuvenation strategies across multiple organs at single-cell resolution. In particular, we leverage network biology approaches to characterize and compare the effects of each strategy at the level of intracellular signaling, cell-cell communication, and transcriptional regulation. As a result, we identified master regulators orchestrating the rejuvenation response and propose that targeting a combination of them leads to a more holistic improvement of age-dysregulated cellular processes. Thus, the interactive database accompanying SINGULAR is expected to facilitate the future design of synthetic rejuvenation interventions.