Utility of microbial cell-free DNA sequencing in the diagnosis of mycobacterial infections in a quaternary care center

2025/01/01 by Fernando H Centeno, Todd Lasco, Ahmed M Hamdi +1 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Cancer Genomics and Diagnostics #Mycobacterium research and diagnosis #Tuberculosis Research and Epidemiology

paper · pdf · doi:10.1017/ash.2025.10057

openalex publication_date 2025/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We examine the performance of microbial cell-free DNA (mcfDNA) next-generation sequencing (NGS) testing on patients admitted to a quaternary care hospital in Houston, Texas. The test was 75.0% sensitive and 97.8% specific for all mycobacterial infections. mcfDNA NGS results led to adjustments in antimicrobial therapy for seven of nine patients with positive results.

Cited by

• Utility of microbial cell-free DNA sequencing in the diagnosis of mycobacterial infections in a quaternary care center by Centeno et al.

Discussions

• In this series, most patients diagnosed with microbial cell-free DNA sequencing see an impact in their antimicrobial management, from starting directed treatment to stopping unnecessary antibiotics. [bsky, 1 points, 0 comments]

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