2026/02/25 by Ian Coghill, Fivos Pham, Mrinal Mandal +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Advanced Fluorescence Microscopy Techniques #Advanced Neuroimaging Techniques and Applications #Optical Imaging and Spectroscopy Techniques
paper · doi:10.1364/opticaopen.31398633
openalex publication_date 2026/02/25 · openalex created_date 2026/02/25 · openalex updated_date 2026/07/15
Spatiotemporal modulation of excitation adds a programmable control layer to fluorescence imaging, enhancing probe functionality and enabling contrast mechanisms and quantitative readouts beyond intensity- or time-resolved approaches. We present two approaches exploiting this control. Modulated Illumination in Space and Time for Optical Sectioning (MIST-OS) suppresses out-of-focus fluorescence using oscillating stripes and temporally resolved detection, while Fourier-Transform Fluorescence Activation and Diffusion Exchange (FT-FADE) applies Fourier-domain analysis to patterned photoactivation for local one-dimensional diffusion measurement. These developments, along with ongoing efforts to establish similar protocols and perform parallelized temporal experiments (also demonstrated here on bacteria), motivated the creation of a custom dual–DMD microscope, fully documented to enable adoption by other laboratories.