2024/10/21 by Baird, Richard G., Apratim Majumder, Majumder, Apratim +2
Biochemistry, Genetics and Molecular Biology · Engineering · #CCD and CMOS Imaging Sensors #FOS: Physical sciences #Image Processing Techniques and Applications #Optics (physics.optics) #Spectroscopy Techniques in Biomedical and Chemical Research
paper · pdf · doi:10.48550/arxiv.2410.15552
openalex publication_date 2024/10/21 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of ∼10\thinspaceμs (∼\thinspace50,000 frames/s), while the CIS provides diffraction-limited spatial resolution. A diffractive optical element encodes spectral information into a diffractogram, which is recorded by the CIS. The diffractogram is processed using a deep neural network to resolve the fluorescence of two beads, whose emission peaks are separated by only 7 nm and exhibit an 88% spectral overlap. We validate our microscope by imaging the capillary flow of fluorescent beads, demonstrating a significant advancement in ultra-fast spectral microscopy. This technique holds broad potential for elucidating foundational dynamic biological processes.