2026/02/07 by Chase A. Weinholtz, Lili Zhou, Vishnu Muraleedharan Saraswathy +8 · 1 voice
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Developmental Biology and Gene Regulation #Neurogenesis and neuroplasticity mechanisms #Zebrafish Biomedical Research Applications
paper · doi:10.64898/2026.02.04.703854
openalex publication_date 2026/02/07 · openalex created_date 2026/02/10 · openalex updated_date 2026/08/01
ABSTRACT Adult zebrafish exhibit full recovery following spinal cord injury. Transient expansion of stem cell-like progenitors is thought to underlie their regenerative capacity. Yet, our understanding of the identities and contributions of the crucial stem cell populations that direct spontaneous neural repair remains limited. Moreover, while most neural regeneration research is centered on promoting proliferative repair, the regulatory mechanisms that reinstate quiescence post-repair are unknown. Here, we determined the molecular identities and cellular contributions of sox2 + progenitors during spinal cord repair. Genetic lineage tracing shows zebrafish spinal progenitors, while quiescent in uninjured tissues, self-renew and differentiate into neurons and glia after injury. By single-cell sequencing, sox2 + cells are heterogeneous and biased towards neuronal or glial fates in both homeostatic and regenerating tissues. By screening for transcription factors that are differentially expressed in acute versus chronic spinal cord injury, we find the Bach1 transcription factors control transient progenitor cell activation by acting as dual activators and repressors of sox2 expression. This study elucidates the molecular diversity and contributions of sox2 expressing cells during spinal cord repair and identifies a transcriptional regulatory switch by which progenitor cells expand after injury and restore quiescence after regeneration is completed.