2026/05/05 by Fitria Ningsih, MAZYTHA KINANTI RACHMANIA, DHIAN CHITRA AYU FITRIA SARI +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Genomics and Phylogenetic Studies #Microbial Metabolism and Applications #Plant Pathogenic Bacteria Studies
paper · doi:10.1099/ijsem.0.007136
openalex publication_date 2026/05/05 · openalex created_date 2026/05/06 · openalex updated_date 2026/07/17
A thermophilic, yellow-pigmented bacterial strain, designated LT1-2-5 T , was isolated from litter in the Cisolok Geyser, West Java, Indonesia. This strain is Gram-stain-negative, aerobic and non-sporulating, with rod-shaped cells and short filaments. Phylogenetic analyses based on 16S rRNA gene sequences (1,446 bp) placed strain LT1-2-5 T within the genus Thermus , showing highest similarities to Thermus thalpophilus SYSU G00506 T (98.54%) and Thermus brockianus YS038 T (98.34%). The strain exhibited optimal growth at 60–65 °C, pH 7.0–8.0 and ≤2.0% (w/v) sodium chloride (NaCl). Major cellular fatty acids (>10%) were iso-C 17:0 , iso-C 15:0 , anteiso-C 15:0 and anteiso-C 17:0 . The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. The major menaquinone was menaquinone-8. The genome size of strain LT1-2-5 T was 2.41 Mbp, and the DNA G+C content was 66.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain LT1-2-5 T and T. thalpophilus SYSU G00506 T were 91.46% and 42.9%, respectively, both of which are below the established species cut-off value. Polyphasic taxonomy, including phylogenetic, genomic, phenotypic and chemotaxonomic analyses, confirmed the status of strain LT1-2-5 T as a novel species of Thermus , for which the name Thermus javaensis sp. nov. is proposed. The type strain is LT1-2-5 T (=UICC B-84 T =CGMCC 1.61921 T =KCTC 102310 T ).