2025/02/28 by Adrijana Crncec, Ho Wai Lau, Lau Yan Ng +5 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Cancer-related Molecular Pathways #DNA Repair Mechanisms #Microtubule and mitosis dynamics
paper · doi:10.1083/jcb.202409219
openalex publication_date 2025/02/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Cyclins and cyclin-dependent kinases (CDKs) orchestrate key events in the cell cycle. However, the uniqueness of individual mitotic cyclins has been a long-standing puzzle. By rapidly removing cyclins in G2 human cells, we found that deficiency of B-type cyclins attenuates mitotic onset and uncouples the G2-M kinase network from mitosis, resulting in sustained activation of PLK1 and cyclin A-CDK1. This culminates in mitotic slippage without completing nuclear envelope breakdown. Remarkably, elevating cyclin A several-fold above its endogenous level is adequate to restore mitosis, allowing cells to survive without B-type cyclins. In contrast, cyclin A is rate-limiting but not essential for G2-M due to compensation by endogenous cyclin B1-CDK2, a non-canonical pair. These findings challenge the traditional indispensable roles of different cyclins and highlight their plasticity. Due to the high malleability of the A- and B-type cyclins, cancer cells may be able to place different weights on different cyclins, while maintaining sufficient CDK activities for successful mitosis.