1985/12/13 by A. Boyde · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Advanced Electron Microscopy Techniques and Applications #Advanced X-ray Imaging Techniques
paper · doi:10.1126/science.4071051
openalex publication_date 1985/12/13 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Stereoscopic pair images with parallel projection geometry are obtained by through-focusing along two inclined axes while recording two (summed and stacked) images with a microscope with a very shallow depth of field. The two stack images sample the same depth slice of translucent or reflective specimens. The method will work most conveniently with a tandem scanning microscope (a direct-view, confocal scanning optical microscope). This is a direct method for recording stereo images that can be used to the limit of resolution in optical microscopy. It demonstrates a previously unrealized advantage of confocal optical microscopy.