2009/06/01 by O. V. Arkhipova, О. В. Архипова, Maria Chuvochina +2
Engineering · Environmental Science · #Anaerobic Digestion and Biogas Production #Microbial Community Ecology and Physiology #Microbial Fuel Cells and Bioremediation
paper · doi:10.1134/s0026261709030060
crossref issued 2009/06/01 · crossref published 2009/06/01 · crossref published-print 2009/06/01 · openalex publication_date 2009/06/01 · crossref published-online 2009/06/07 · crossref created 2011/04/28 · openalex created_date 2025/10/10 · crossref deposited 2026/03/29 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/31
Cytochromes c were found in the cells of the bacterium Geobacter sulfurreducens AM-1 grown on acetate and methacrylate. The periplasmic extract of G. sulfurreducens AM-1 contained about 88% of the total content of cytochromes c of intact cells. The analysis of cytochromes c from the native cells of G. sulfurreducens AM-1, from the periplasmic extract and from the cells treated by an alkaline solution showed the presence of nine proteins containing heme c. The molecular masses of cytochromes c from G. sulfurreducens AM-1 were 12.5, 15.5, 25.7, 29.5, 34.7, 41.7, 50.1, 63.1, and 67.6 kDa; localization of each cytochrome c was determined. Three heme-containing proteins (15.5 kDa, 25.7 kDa, and 29.5 kDa with the most intensive staining) were present mainly in the periplasm of the bacterium. The other two (50.1 and 67.6 kDa) were supposedly localized in the cell membrane. Cytochromes c with the molecular masses of 12.5, 15.5, and 67.6 kDa are considered as possible components of the methacrylate redox system of G. sulfurreducens AM-1.