2024/06/12 by Kohei Otomo, Takaki Omura, Yuki Nozawa +41 · 1 voice · 3 citations
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques #Cell Image Analysis Techniques #Single-cell and spatial transcriptomics
paper · pdf · doi:10.1038/s41467-024-49131-1
openalex publication_date 2024/06/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost (20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.