2025/07/08 by Yashika Bansal, A. Mujib, Jyoti Mamgain +4 · 1 voice
Chemistry · Medicine · #Chemical synthesis and alkaloids #Fungal Biology and Applications
paper · doi:10.21273/hortsci18602-25
openalex publication_date 2025/07/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
Crinum asiaticum is an important medicinal and ornamental plant known for its anticancer, analgesic, antiviral, anti-inflammatory, and antifungal properties attributable to its diverse classes of compounds. However, the plant is being overexploited in its native habitat because of its therapeutically significant phytocompounds, leading to a decline in its population. Thus, this study aimed to establish a callus culture system and detect and compare the metabolites content in both in vivo and in vitro regenerated calli. In vitro callus culture of C. asiaticum was initiated from bulb scales on Murashige and Skoog medium supplemented with different concentrations of plant growth regulators. Afterward, gas chromatography-mass spectrometry (GC-MS) analyses of methanol extracts of bulb scale and bulb scale-derived calli were performed. The antioxidant potentials of both the samples were evaluated by determining the 2, 2-diphenyl-1-picrylhydrazyl (DPPH), peroxidase (POD), and superoxide dismutase (SOD) activities. The medium fortified with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D) produced profuse callus with a frequency of 95.83%. The GC-MS analysis of both extracts confirmed the presence of 34 and 31 bioactive compounds, respectively. Various phytochemicals such as gamma-sitosterol, squalene, vitamin E, linolenic acid, and guanosine were present in both extracts. The results of all the anti-oxidant assays (DPPH, POD, and SOD) revealed higher antioxidant activities in callus compared with those in bulb scale tissue. This study proved the potential of using callus culture on a larger scale as a source of several important secondary metabolites in the pharmaceutical industry.