2023/10/16 by Ashwin J. X. Choorakuttil, Andrea Pruccoli, Martin Winterhalder +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Materials Science · #Advanced Fluorescence Microscopy Techniques #Gold and Silver Nanoparticles Synthesis and Applications #Spectroscopy Techniques in Biomedical and Chemical Research
paper · pdf · doi:10.1063/5.0171725
openalex publication_date 2023/10/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/08
We report an experimental scheme for stimulated Raman scattering (SRS) microscopy with excitation in the visible spectral region. This allows electronically preresonant (epr) SRS microscopy of a broad range of chromophores with sensitivities as low as 1 μM. Our experiment is based on two synchronously near-infrared pumped optical parametric oscillators (OPO). One of the outputs is modulated at a fourth of the repetition rate with a novel broadband electro-optical modulator. Using a combination of spectral focusing and tuning of the OPO, we show the recording of epr-SRS spectra over the whole range of molecular vibrations at a speed up to 20 times faster than classical wavelength tuning. The imaging capabilities of this setup are demonstrated with material scientific and cellular samples.