2026/04/17 by Keith Lyons, Dayle Leonard, Leona McSharry +6 · 1 voice
Biochemistry, Genetics and Molecular Biology · Earth and Planetary Sciences · #Ion channel regulation and function #Marine Invertebrate Physiology and Ecology #Venomous Animal Envenomation and Studies
paper · doi:10.1016/j.toxcx.2026.100256
openalex publication_date 2026/04/17 · openalex created_date 2026/04/18 · openalex updated_date 2026/07/23
Historically, venom potencies have been assessed using measures of lethality, such as the median lethal dose (LD 50 ). However, venoms may be selected primarily for their ability to rapidly incapacitate rather than cause mortality, meaning LD 50 may not capture the efficacy of venoms in an ecological and evolutionary context. To capture this context, recent studies have adapted measures that assess venoms’ ability to rapidly incapacitate, such as the median paralysis dose (PD 50 ). However, while PD 50 values are expected to provide a more proximate assessment of ecological variation in venom potency, it is unknown whether historically available LD 50 values are still useful proxies of ecologically relevant potency or whether they capture independent axes of venom variation. Here, we test the relationship between LD 50 and PD 50 in spider venoms by experimentally estimating LD 50 and PD 50 for 12 species and collating additional potency data for 46 species retrieved from the literature, producing a dataset of 55 species spanning 26 families when combined. We observed a linear isometric relationship between LD 50 and PD 50 , showing these potency measures are both strongly correlated, with an increase in paralysis efficiency associated with a similar increase in lethality. Our results suggest that due to the correlation between functional aspects of venom potency, paralysis and lethality, historically available LD 50 values may be used to compare general venom potencies in spiders, provided that they are based on the same prey model. • We found an isometric relationship between PD 50 & LD 50 in 55 spider species venoms. • The main analysis included data produced in the lab & compiled literature data. • Spider venom PD 50 & LD 50 values may be used as proxies for one another.