2001/10/27 by M. A. Kiselev, P. Lesieur, A. M. Kisselev +3
Physics and Astronomy · Biochemistry, Genetics and Molecular Biology · #physics.bio-ph #cond-mat.soft #physics.chem-ph #q-bio
published as Journal of Alloys and Compounds v. 328 (2001) p. 71-76 · PDF: 13 pages, 6 figues. MS Word sourse
arxiv created 2001/10/27 · arxiv updated 2009/11/30
The possibility to use sucrose solutions as medium for X-ray and neutron small-angle scattering experiments has been explored for dimyristoylphosphatidylcholine (DMPC) vesicles and mixed DMPC/C_(12)E_(8) aggregates. The influence of sucrose concentration on phospholipid vesicles size and polydispersity has been investigated by complimentary X-ray and neutron scattering. Sucrose solutions decreased vesicle size and polydispersity and increased a contrast between phospholipid membrane and bulk solvent sufficiently for X-rays. 40% sucrose in H2O increased X-ray contrast by up to 10 times compared to pure H2O. The range of sucrose concentration 30%-40% created the best experimental conditions for the X-ray small-angle experiment with phospholipid vesicles.