PCR differentiation of Escherichia coli from other Gram-negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein

1998/12/01 by J. Chen, M. W. Griffiths, Mansel W. Griffiths · 1 citation
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #Molecular Biology Techniques and Applications #Salmonella and Campylobacter epidemiology

paper · pdf · doi:10.1046/j.1472-765x.1998.00445.x

openalex publication_date 1998/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30

Abstract

A PCR-based test was developed to differentiate Escherichia coli from other Gram-negative bacteria. The assay employed primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein (uspA) and was found to be highly specific for E. coli. An 884 base pair (bp) specific product was amplified from all of the E. coli tested (n = 45), whereas no amplification was associated with non-E. coli Gram-negative bacteria (n = 11).

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